Fertility over 40 - Age-Related Infertility

by Samuel C. Pang, MD., Associate Medical Director Reproductive Science Center, Lexington, MA

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Many People are aware that the fertility potential of a woman declines with increasing age. Most, however, are not aware of how soon fertility begins to decline. The fertility potential of the average woman begins to decline appreciably at the age of 35 years, and begins to decline dramatically beyond the age of 40 years. This article will discuss the main factors that contribute to the decline in fertility potential of women with increasing age, as well as some of the assessment tests and treatment options. There are two main reasons that explain the decline in fertility potential of women beyond the age of 40 years. The first is the phenomenon of chromosomal aneuploidy and the second is the concept of decreasing ovarian reserve.

Chromosomal Aneuploidy
This term refers to the abnormal number of chromosomes in the egg or the embryo. As a woman ages, an ever-increasing proportion of the eggs she releases contain an abnormal number of chromosomes. Eggs are produced in the ovary by cell division, and the older a woman gets the more likely there are to be mistakes in the process of cell division such that the egg ends up with an abnormal number of chromosomes. A theory that has been proposed to explain this phenomenon suggests that this occurs because the eggs being released by an older woman have gone through multiple generations of cell division, and with each generation of cell division, the likelihood of error in distribution of chromosomes increases.

Chromosomal aneuploidy accounts for three consequences in the reproductive efforts of women who are 35 years of age or older. The first is the well- known phenomenon of higher risks of having a baby with Down Syndrome with increasing age beyond 35 years. However, this is simply the tip of the iceberg. Chromosomal aneuploidy also causes the higher risk of spontaneous miscarriages in women with increasing age beyond 35 years. Down Syndrome is the result of Trisomy 21, which causes relatively mild abnormalities in the fetus. Aneuploidies involving most of the other chromosomes typically result in more severe abnormalitites of the fetus, which will result in spontaneous miscarriage. There are also chromosomal aneuploidies that result in such severe abnormalities of the embryo such that the embryo does not even implant to result in pregnancy. This explains why it becomes progressively more difficult for women to conceive with increasing age beyond 35 years. In summary, the phenomenon of chromosomal aneuploidy explains the increasing difficulty to conceive, the increasing risk of spontaneous miscarriage, as well as the increasing risk of having a baby with Down Syndrome of other chromosomal anomalies.

Ovarian Reserve
All women are born with a genetically pre-determined lifespan to their ovarian function. The average woman's ovaries stop functioning at the age of 50 or 51 years. This event is referred to as the menopause. However, there are some normal women whose ovaries stop functioning as early as 40 years, and others whose ovaries may continue to funtion into their late 50's. Environmental factors or disease conditions may shorten the lifespan of a woman's ovaries. Smoking cigarettes is one of the most common and important factors that has been found to decrease ovarian reserve. Other examples of environmental factors that may decrease ovarian reserve include exposure to chemotherapy agents or radiation therapy used in the treatment of cancer. Disease conditions of the ovaries, such as ovarian endometriomas (ovarian cysts caused by endometriosis), may require surgery to remove the endometriomas, which can destroy ovarian tissue, and may result in decreasing ovarian reserve.

The ovarian reserve of each woman generally begins to decline about 15 years prior to menopause. This reflects a diminishing supply of eggs in the ovaries, and is manifested by an increase in Follicle Stimulating Hormone (FSH) and Estradiol (E2) levels in the blood during the time of menstruation. A test commonly used to evaluate a woman's ovarian reserve is the Clomiphene Citrate Challenge Test (CCCT). The FSH & E2 levels in the blood are measured on cycle day (CD) #3 of the menstrual cycle, following which the woman takes clomiphene citrate 100 mg daily form CD#5 to CD#9. The FSH & E2 levels are repeated on CD#10. The CCCT is considered normal if the FSH levels on CD#3 and CD#10 are 12 mIU/mL or less. The CCCT is generally considered abnormal if the E2 level is higher than 75 pg/mL on CD#3 or if the FSH level on either CD#3 or CD#10 is higher than 12 mIU/mL. (These thresholds may differ depending on the laboratory in which the tests are done. Each fertility center has usually established their own threshold levels based on their own laboratory and clinical outcome data, so it may not be appropriate to interpret results of these tests done in the laboratory of one fertility center using the threshold levels established by another fertility center.)

Another blood hormone test that has been proposed for assessing ovarian reserve is Inhibin B. In theory, a low level of inhibin B on CD#3 suggests decreased ovarian reserve. However, the threshold level which divides "normal" and "abnormal" is not well defined, and some studies have suggested that inhibin B may not be a very useful test for measuring ovarian reserve.

Another blood hormone test that is currently being evaluated for its potential usefulness in measuring ovarian reserve is the Anti-Mullerian Hormone (AMH), which have also been referred to as the Mullerian-Inhibiting Substance (MIS) or Factor (MIF). Although this test holds some promise, it is still being tested experimentally, has not been validated for assessment of ovarian reserve, and is not yet commercially available for use.

Another method of evaluating ovarian reserve, which does not involve testing blood hormone levels, involves ultrasound evaluation of the size of the woman's ovaries and the number of measurable small (antral) follicles during the early follicular phase of the cycle (which is typically during mentration). This test has been used by some physicians to predict ovarian response to gonadotropin stimulation.

Unfortunately, decreased ovarian reserve is a condition that cannot be reversed with medical treatment. Despite major advances in medical technology, no magic pill has been invented which reverses the aging process of the ovaries. Therefore, the first-line option for dealing with decreased ovarian reserve is using very high doses of gonadotropins to stimulate the ovaries, together with various novel protocol strategies which may (or may not) improve ovarian response in some women. Some of these protocol strategies may involve using pre-treatment with oral contraceptive pills, using a specially diluted dosage of Lupron (often referred to as the Lupron Microdose protocol), using an antagonist protocol, Cetrotide or Antagon, using a combination of clomiphene and gonadotropins, etc. If none of these strategies is successful, then the remaining option for achieving a successful pregnancy is the use of donor eggs.

The above article is reprinted with permission from RESOLVE: The National Infertility Association.

See a fertility specialist now
Don't wait, see a specialist (reproductive endocrinologist) for a consultation. We're a national network of over 100 fertility specialists.

Samuel Pang, M.D., is an infertility specialist with the Reproductive Science Center, a state-of-the-art fertility clinic serving Boston and New England.

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